nest-PCR

基本解释

Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established.
获取正常男性单个淋巴细胞和无DMD家族史的单个胚胎细胞,行DMD基因外显子17、19、44、45、48的五重巢式PCR,分析扩增成功率、假阳性率和假阴性率。
Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced.
方法以正常人肺组织mRNA为模板 ,采用逆转录反应 (RT) PCR、巢式 PCR及基因克隆技术 ,获得pUC19/SP A 6A克隆 ,并测序证实。
Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+).
方法通过巢式PCR,从一份兰州地区无偿献血员血清中扩增TTV病毒的ORF2基因,并克隆到pGEM-T载体中,进行核苷酸序列分析。
X gene (HBx), enhancer II (Enh2) and basic core promoter (Bcp) of HBV in sera from HCC patients were amplified by nest PCR , and sequenced and then compared with the sequences in Genbank.
对HCC患者血清的HBV的HBx、Enh2与Bcp基因的巢式PCR产物直接测序，与Genbank中已发表序列进行对比分析。
Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR.
利用套式PCR技术检测母血中胎儿SRY基因的研究